Everything You Need to Know About CRISPR-Cas9

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology is a simple but powerful tool for genome editing. This tool enables life science researchers to easily edit DNA sequences and modify gene function. It has many potential applications include correcting genetic defects, treating and preventing the spread of diseases and improving crops. By delivering the CRISPR enzyme Cas9 nuclease coupled with synthetic guide RNA (gRNA) into a cell, the cell's genome can be cut at a desired location, that allows existing genes to be removed or add new ones.

Increasing usage of CRISPR systems in microbiology, growing government and private investments on research and development of genome editing, rising prevalence of genetic disorders, and increases application of CRISPR/Cas9 technology to improve crop production drives the global CRISPR technology market. However, ethical issues associated with CRISPR and lack of skilled personnel restrain the global CRISPR technology market over the forecast period.

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Dominated Players in the CRISPR Technology

Are Thermo Fisher Scientific, Merck KGaA, Horizon Discovery Group, Cellecta, GeneCopoeia,New England Biolabs, OriGene Technologies, GenScript, Integrated DNA Technologies,and Agilent Technologies

CRISPR-Cas9 Mechanism

The beauty of CRISPR lies in its simplicity. CRISPR relies on just two components: the molecular scissors, a CRISPR-associated (Cas) nuclease, and the GPS guiding it to the appropriate site, the guide RNA (gRNA). In natural, the gRNA consists of two distinct segments of RNA: CRISPR RNA (crRNA) and transactivating CRISPR RNA (tracrRNA). Synthetically, they can be engineered as one seamless fusion sequence known as single guide RNA (sgRNA). This section of our guide digs into how CRISPR-Cas9 works.

The global CRISPR technology market is segmented on the basis of product and services, application, end user. Based product and services, the market is segmented as, enzymes, kits, services and others. The CRISPR technology market is categorized based on application into, genetic engineering, cell line engineering and others. Based on end user, the CRISPR Technology market is classified into biotechnology & pharmaceutical companies, contract research organizations (CROS), and academic & government research institutes.

Now that CRISPR has gained popularity within the scientific community, there is a surplus of information surrounding this technology. In fact, it can be daunting to scour the internet to identify the useful resources to learn about CRISPR-Cas9. We have compiled the most valuable information to serve as a one-stop resource for all your CRISPR needs. Whether you want to brush up the basics of CRISPR, need help with scientific details of CRISPR gene editing experiments, or are simply curious about its real-world applications, we have covered everything!

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References:

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