Bioinformatics Tips Next generation Sequencing Data Processing

Experience powerful NGS data analysis capabilities

Next generation sequencing (NGS) has created a noteworthy paradigm shift in the clinical diagnostic field. It refers to an aggregate collection of methods in which various sequencing reactions occur at the same time, bringing about vast amounts of sequencing data for a little division of the cost of Sanger sequencing.

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The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes, of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species.

Applications of NGS

NGS technologies have many applications such as DNA-sequencing and assembly to determine an unknown genome without any preparation or search for variations among genome samples, RNA-sequencing, to analyze gene expression and to predominantly identify DNA regions of DNA binding proteins, for example, transcription factors etc.

NGS-based diagnostics implement some portion of the clinical genomic testing in which a limited set of genes are targeted and not the entire genome and exome.

DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.

Knowledge of DNA sequences has become indispensable for basic biological research, and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. Comparing healthy and mutated DNA sequences can diagnose different diseases including various cancers,characterize antibody repertoire, and can be used to guide patient treatment. Having a quick way to sequence DNA allows for faster and more individualized medical care to be administered, and for more organisms to be identified and cataloged.

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