Rapid microbiology testing is done for the faster identification of microbial risks, monitoring critical control points in real time, contribution for the improvement, manufacturing processes, enhancing safety profile of products and also decreases overall costs of quality programs. This technique is widely used in for the research activity, drug development and others. It ensure testing in rapid and accurate manners at reasonable costs. The ideal test should achieve detection of certain types of microorganisms that are undetected by the pharmacopoeia-defined sterility tests and its accuracy should be confirmed by regulatory authorities.